Easy to Apply Instructions

It is very easy to set up a method in the Beckman P/ACE 5000 series instrument. Using the following parameters, fill your vials and enter this information into instrument for best results.

Fill your vials as follows

1. Vial# 10 – waste
2. Vials# 4, 33, 34 – CElixir Accelerator (B) with the pH of your choice
3. Vials# 29, 31 – CE grade Water
4. Vial# 32 – CElixir Initiator (A)
5. Vial# 30 – 0.1 NaOH
6. Other Vials – Samples

1. Rinse the capillary using pressure for 5 minutes with 0.1N NaOH.
2. Rinse the capillary using pressure for 1 minute with CEwater vial# 29.

3. With pressure, rinse the capillary from vial#32 with the outlet vial# 10 (forward high 20 p.s.i.) for 0.20 minute. This is the Initiator (A) Step.
4. With pressure, rinse the capillary from vial#33 with the outlet vial#10 (forward high 20 p.s.i.) for 0.50 minute. This is the first Accelerator (B) Step for coating.

5. Inject your sample using pressure for 5 seconds with the outlet vial#4.
6. Inject a “Water Plug” behind your sample. For 1.0 second, using pressure, inject CEwater behind your sample for injection precision and a possible stacking effect. Inlet is Vial#31 and outlet vial is #4.

7. Run your separation, for example 25kV for 15.0 minutes to be adapted in function of capillary length and observed µAmp. It is important to separate using inlet Vial#34 and the outlet Vial#4 during the run. Use 1 minute ramping time.
8. Use the AutoZero function for example after 2 minutes.
9. Stop Collecting data at the end of your run, for example 15.0 minutes.

10. Using pressure, run 0.1N NaOH through the capillary using Inlet Vial#30 and Outlet Vial# 10 for 0.50 minutes (Forward High)
11. Using pressure, run CEwater through the capillary using Inlet Vial#29 and Outlet Vial# 10 for 0.50 minutes (Forward High)

Be sure to have a maximum free space and a low level of fragmentation of the files on the hard disk.