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Biomolecular Separations:
Lactoglobulin and Trypsin Inhibitor Standards
Method Conditions:
Voltage: 30 kV
Capillary: MicroSolvCE Bare Fused Silica 50µm 60cm Total
Injection: Hydrodynamic, 7mbar, 5 seconds
Run Buffer: CElixir Accelerator (B) pH 6.2
Organic Additive: None
Detection: UV 190nm |
Click here for catalog description of CElixir
Method:
Using a Beckman P/ACE MDQ capillary electrophoresis instrument and a MicroSolvCE bare fused silica
capillary, catalog number 04050-C, the separation of both Lactoglobulin and Trypsin Inhibitor protein standards was
shown. Ramping the voltage for one minute before beginning the separation was employed after inserting a 1 sec
injection of a “water plug”. This aided in the precision of this method.
Discussion and Rationale:
The blue e-gram is Trypsin Inhibitor standard and the red e-gram is the Lactoglobulin Protein standard. These
separations show that separation near neutral pH produces very good peaks.
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