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How To Change Columns from Reverse Phase to Normal Phase Mode and Vice Versa
Just as a reminder, all Cogent Bidendate C18 and C8 columns are filled with water containing solvents during shipping. To start work in Normal Phase Mode, a simple switching procedure is recommended. A simple 30 minute procedure allows switching from one mode to another. Procedure: A – moving from Reverse Phase to Normal Phase HPLC; pump 100% methanol for 15 minutes at 1 mL/min. flow rate, followed by 15 minutes 100% methylene chloride. The column is ready to be equilibrated with mobile phase for NP-HPLC. B – moving from Normal Phase to Reverse Phase HPLC; pump 100% methylene chloride for 15 minutes at 1 mL/min. flow rate, followed by 15 minutes 100% methanol. The column is ready to be equilibrated with mobile phase for RP-HPLC.
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