MicroSolv Technology Corporation

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Frequently Asked Questions About Cogent™ HPLC Columns

Why buy and use Cogent™ HPLC Columns?

Who is the manufacturer of the silica that is used in Cogent™ HPLC columns?

What is the maximum pressure Cogent HPLC columns will tolerate?

What is normal volume of Cogent HPLC Columns?

What is the advantage of using Normal Phase over Reversed Phased HPLC?

Most acids and bases are not effective when the organic content of the mobile phase goes too high. Since I am interested to use the Cogent columns in ANP, what can I do to adequately "acidify" the mobile phase when organic is higher than 40%?

1. Why buy and use Cogent™ HPLC Columns? [top]

Reliability
Excellent peak shapes
Powerful and Easy to Use
Stable
Lower Cost Compared to Comparable Columns

Manufactured with state of the art technologies, Cogent™ columns are becoming the choice of many advanced HPLC laboratories around the world.

The Cogent™ brand of HPLC columns have a wide range of performance options ranging from irregular, 10µm silica based stationary phases for historic methods to the advanced TYPE-C™ silica based columns for what were once impossible separations. All Cogent columns are tested and the actual test chromatogram is supplied.

All Cogent™ Columns are packed at very high pressure in only Isobore™ tubes. These column tubes have a patented passivation technology which further enhances a good packed column with additional efficiency.

Buy Cogent e-Columns for traditional method development. These type-B high purity silica phases are mono-functionally bonded and are packed to provide maximum value. These columns are double end-capped when appropriate.

Buy Cogent HPS™ columns when you need the most reliable performance for separations and peak shape. Used widely for pharmaceutical applications, these reliable columns are tri-functionally bonded on our highest purity silica and lowest metal content. The particle size distribution is very tight and offers the highest in efficiency and reliability. These columns are double end-capped when appropriate. All the bonded phases are manufactured under strict anaerobic conditions and unique bonding techniques have been employed. For this reason the bonded phases are covered uniformly on the silica surface.

Buy Cogent hQ™ columns when you need reliable performance for slightly polar compounds and when the Cogent HPS™ or comparable products such as Luna®, Symmetry™, Inertsil™ and other market leaders will not separate these compounds. Based on the same High Purity Silica as the Cogent HPS™ these unique columns are not end-capped. The Cogent hQ™ C8 and C18 offer chromatographers a very powerful tool in the lab that are not available from most suppliers.

Buy Cogent TYPE-C™ Silica based HPLC Columns for difficult separations or when you need to use method extremes with other columns. These columns are based on our High Purity, low metal content Silica and are not end-capped. Using our unique patented technology these columns offer little to no surface silanols. The mobile phase will solvate these columns very differently from traditional HPLC phases. One column will produce efficient, reproducible peaks in Reverse Phase, Normal Phase or Aqueous Normal Phase (Normal Phase elution and separation but using Aqueous Based Mobile Phases). Separate polar and non polar compounds in the same isocratic run, swap peak order by simple changes in mobile phase A:B ratios and many other powerful uses.

2. Who is the manufacturer of the silica that is used in Cogent™ HPLC columns? [top]
MicroSolv Technology is the sole source and producer of the silica used in HPS™, UDC-Cholesterol, e-Columns™ and hQ™ product lines.

3. What is the maximum pressure Cogent HPLC columns will tolerate? [top]
Cogent HPLC Columns are rated up to 6,000 psi. It is not recommended by most instrument producers that analyses are carried out at this high level.

4. What is normal volume of Cogent HPLC Columns? [top]
Use this chart for approximate answers to that question:

Calculate Your Own 4.6mm ID
50mm Long
75mm Long
100mmLong
150mmLong
250mmLong

3.0mm ID
50mm Long
150mmLong
250mmLong
Volume
0.83ml
1.25ml
1.66ml
2.49ml
4.15ml

Volume
0.353ml
1.060ml
1.766ml

5. What is the advantage of using Normal Phase over Reversed Phased HPLC? [top]
Any advantage would be dependant on your sample and your separation goals. Generally, Normal Phase HPLC is better at separation of polar compounds then Reversed Phase.

6. Most acids and bases are not effective when the organic content of the mobile phase goes too high. Since I am interested to use the Cogent columns in ANP, what can I do to adequately "acidify" the mobile phase when organic is higher than 40%? [top]
We have had some good success with methyl phosphonic acid. It remains soluble in acetonitrile content as high as 96%. This might help when some compounds are retaining in ANP but the peak shape needs to be tightened up a bit.

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