Frequently Asked Questions about
ß-Cyclo-100 ChiralCE Columns for HPCE
- How do the ß-Cyclo-100 Columns enhance chiral effectiveness?
- At which pH will the ß-Cyclo-100 columns be stable?
- What column ID does ß-Cyclo-100 come in?
- Can I use the MicroSolvCE Window Maker™ or similar units on the ß-Cyclo-100?
- Do I have to use Cyclodextrins in my run buffer to achieve a chiral separation?
- What does the coating of the ß-Cyclo-100 do to the EOF?
- How do I increase the solubility of my ß-Cyclodextrin additive in my buffers?
- How does the ß-Cyclodextrin resolve enantiomers?
1. How do the ß-Cyclo-100 Columns enhance chiral effectiveness? [top]
We permanently bond to the bare fused silica wall, permethylated ß Cyclodextrin using a moderately polar base
coating. Not only does this “coat” the wall and prevent competing equilibria, it provides additional inclusion
complexation for enhanced effectiveness. A slower EOF than bare fused silica will allow enantiomers and other
isomers more time in the “chiral” environment and shift what the causes migration more to electrophoretic mobility
away from EOF.
2. At which pH will the ß-Cyclo-100 columns be stable? [top]
The ß-Cyclo-100 is generally stable between pH 2 through 9.
3. What column ID does ß-Cyclo-100 come in? [top]
The ß-Cyclo-100 is available in both 50m and 75m ID width. The length of the columns are 1 meter.
4. Can I use the MicroSolvCE Window Maker™ or similar units on the ß-Cyclo-100? [top]
No, it is not recommended that one use high heat to remove the polyimide coating on the column. This could create a
“void” in the wall coating and produce erroneous results. It is best to use chemicals to remove the polyimide. Be
sure to follow good laboratory practices when using hazardous chemicals.
Is the conditioning of the ß-Cyclo-100 the same as with a bare fused silica capillary?
No. Since the methods and buffers for each run are going to be user specific, the column conditioning will be
varied. Remember that when you create your method that you treat the column with consideration of the Cyclodextrin
coating.
5. Do I have to use Cyclodextrins in my run buffer to achieve a chiral separation? [top]
No. Many scientists have found that additives are not needed. Others have found that their method needed
Cyclodextrin in the run buffer to separate their enantiomers.
6. What does the coating of the ß-Cyclo-100 do to the EOF? [top]
The EOF will generally be slower than a bare fused silica. This slower EOF gives your enantiomers more time to form
the inclusion complex that creates the needed differences in the charge to mass ratio or the affinity for the
cathode.
7. How do I increase the solubility of my ß-Cyclodextrin additive in my buffers? [top]
The reported way to most efficiently increase the solubility of ß Cyclodextrin is to add about 30% ethanol to
your buffer.
8. How does the ß-Cyclodextrin resolve enantiomers? [top]
Two enantiomers that have different affinity to form a complex with ß-Cyclodextrin will form diastereoisomers
while in the capillary environment that is filled with ß-Cyclodextrin. When the inclusion complex or
diastereoisomers are formed in the capillary, they will migrate to the detector at different rates because they will
have different charge to mass ratios. Therefore it is easy to exploit the electrophoretic differences that now exist.
