These 1-meter capillaries are coated with polyethylene glycol and is weakly hydrophilic. This coating exhibits a low hydrogen bonding capacity therefore an excellent choice for hydrophobic proteins and peptides. They are stable in a wide pH ranging from 2 to 10. They are durable and should give many hours of life if used according to the products specifications.

Specifications:
The pH range of the PEG-100 HPCE Columns is 2-8.5
Column ID: 50µ or 75µ
Column OD: 360µm
Capillary Exterior Coating: Polyimide
Length: 1 meter
Coating: Polyethylene Glycol, cross linked.
Surface: Weakly Hydrophilic

Literature References: Zhongxi, Zhao, Adul Malik, Milton Lee, Anal. Cheml, 65,   2747-2752 (1993) S. Terabe, et.al. High Resolu. Chromatogr., 9:667   (1986) G. Bruin, J.P.Chang, R.H. Kuhlman, K. Zegers, J.C. Kraak   and H. Poppe, J. Chromatgr. 471:429 (1989)

Column Conditioning Procedure:

1. Carefully remove the capillary from the shipping/storage container. It is fragile.
2. Remove protective polyimide coating by using 98% fuming sulfuric acid. One drop should be sufficient for complete removal. A low temperature flame may be used as well. Always use appropriate and approved safety precautions when performing the above.
3. Condition the Column by running your “run buffer” through the capillary for 30 minutes.
4. Rinse the column (after step 3) with CEwater™ for 5 minutes.
5. Final rinse should be with your run buffer for an additional 10 minutes.
6. Repeat this procedure between each injection with reduced run times.

1. Rinse the column for 25 minutes with your run buffer.
2. Rinse the column with CEwater™ for fifteen minutes.
3. If storing for an extended period, we recommend that N2 or He should be blown, under slight pressure, through the column.