Amino acid separation:underivatized by LCMS

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Amino Acid Separation without Derivatizing

This figure shows the extracted ion chromatogram (EIC) of a nineteen amino acid mixture under gradient conditions.

Click on Chromatogram to enlarge

Method Conditions and Sample Preparation Protocol

From our experience with amino acid separations in the ANP mode of separation, samples should be prepared as follows:

Stock sample solution (if used) should be prepared in 100% water with 0.1& formic acid (or acetic acid: depends which acid is used in your mobile phase).
Samples used for LC-MS should be diluted using 60% acetonitrile + 0.1%formic (or acetic acid) /40% DI water +0.1% formic (or acetic);

If the stock sample solution is not used sample should be prepared as in point 2 above.

Method Run Conditions:
Column: 2.1 x 150 mm Cogent Diamond Hydride™
Solvent A: DI water + 0.1% formic (or acetic) acid
Solvent B: Acetonitrile + 0.1% formic (or acetic acid)
Flow rate: 0.4 mL/min
Gradient conditions:
     0 - 5 min start at 100%B
     5 - 6 min to 90% B
     6 - 7 min hold 90% B
     7 - 9 min to 70%B
     9 - 10 min hold 70%B
     10- 18 min hold 100%B

     2 min hold 100%B (for equilibration)

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