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a1-acid-glycoprotein
From Sheep
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printable Application Sheet
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Notes: Glycoproteins are macromolecules composed of a polypeptide covalently bonded to a carbohydrate molecule. The sugar can
make up anywhere from less than 1 percent to 80 percent of the total mass of a glycoprotein.
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Method Conditions
| Column |
Cogent Bidentate C8 300™, 5µm, 300A |
| Catalog No. |
40008-75P-3M |
| Dimensions |
4.6 x 75 mm |
| Solvents |
A: DI water + 0.1% trifluoroacetic acid (TFA)
B: acetonitrile + 0.1% TFA |
| Gradient |
| Time |
%B |
| 0.0 |
15 |
| 20.0 |
50 |
| 25.0 |
50 |
| 25.1 |
15 |
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| Post time |
5 min |
| Flow rate |
0.5 mL/min. |
| Sample Peak |
a1-acid-glycoprotein, sheep, 104H9320 0.5 mg/mL in DI water
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| Detection |
UV 214 nm |
| Injection |
1 microL |
Discussion
Glycoproteins have very diverse functions in the living organism. They are used as protective agents, lubricants and they are
constituents of the cell wall. This diversity is a direct function of their structure. A simple, linear Reverse Phase gradient used in
this note shows the retention of sheep glycoprotein. The resulting chromatograms are very reproducible and the equilibration time
between gradient runs is only 5 minutes.
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