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HPLC Application Sheet
Retain Highly Polar Compounds: Analysis of Amino-Caproic Acid by a Simply LC-MS Method
Note: Mobile phase with 0.1% formic acid can be used in the UV detection of 6-amino-n-caproic acid at 206nm.
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printable Application Sheet
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Method Conditions
| Column |
Cogent Bidentate C18, 4µm, 100A |
| Catalog No. |
40018-75P |
| Dimensions |
4.6 x 75mm |
| Mobile Phase |
| A: |
70:30 acetonitrile/DI water 0.1% formic acid |
| B: |
80:20 acetonitrile/DI water 0.1% formic acid |
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| Flow rate |
0.5 mL/minute |
| Injection Volume |
2 µL |
| Peaks |
| 1. |
Uracil m/z 112 |
| 2. |
6-amino-n-caproic acid m/z 132 |
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| Detection |
Atmospheric Pressure Chemical Ionization in positive mode: APCI+. Single Ion Monitoring |
Discussion
6-amino-n-caproic acid is an active pharmaceutical ingredient used in massive thrombolysis and proteolysis
secondary to metastatic carcinoma of the prostate.
It is also a potent in vitro inhibitor of fibrinolysis. After oral administration 6-amino-n-caproic acid
enhances the uptake of labeled fibrinogen in both the Walker and Murphy tumors. The drug is also used in
treatment of ulcers and many other diseases. Chromatograms presented (A and B) show the retention of this
basic molecule using a simple isocratic LC-MS method and a Cogent Bidentate C18 column under Aqueous Normal
Phase conditions. Observe that when the concentration of acetonitrile increases the retention of
6-amino-n-caproic acid also increases.
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