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Aromatic Amino Acids
Separation of L - (+)-alpha-phenylglycine and L-phenylalanine using a simple LC-MS compatible mobile phase. UNDERIVATIZED
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printable Application Sheet
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Note: Alpha – amino acids are precursors for many important chemical entities (for example isoindolines), which are
essential to the discovery of new drugs.
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Method Conditions
| Column |
Cogent Silica-C™, 4µm, 100A |
| Catalog No. |
40000-75P |
| Dimensions |
4.6 x 75 mm |
| Solvents |
A: DI water + 0.1 % formic acid
B: acetonitrile + 0.1% formic acid |
| Mobile Phase |
80%B/20%A |
| Flow rate |
1.0 mL/minute (t0 = 0.85 min) |
| Peaks |
1. L-(+)-alpha-phenylglycine
2. L-phenylalanine |
| Injection Volume |
2 µL |
| Sample Matrix |
0.3 mg/mL of each sample dissolved in 50% acetonitrile/50% DI water + 0.5% formic acid. |
| Detection |
254 nm UV |
Discussion
A Cogent Silica-C™ column was used to separate two important amino acids: L-(+)–alpha-phenylglycine and L-phenylalanine.
There is still an unfulfilled need for rapid and precise determination of amino acids in many types of samples. Ordinary
columns used today such as C18 which are present in every analytical laboratory are not able retain underivatized amino
acids. They usually elute at or near the “dead volume” with all other polar compounds. Cogent Silica-C™ columns
can retain all amino acids (underivatized) including the two presented above.
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