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Aspirin & Salicylic Acid
Excellent Peak Shape For a Very Difficult Compound
Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-75P |
| Dimensions |
4.6 x 75 mm |
| Mobile Phase |
48:52 Acetonitrile: DI Water + 0.1% Phosphoric Acid |
| Flow rate |
1.5 mL/min. |
| Peaks |
1. System Peak
2. Aspirin (Acetylsalicylic Acid)
3. Salicylic Acid
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| Injection Volume |
10 µL |
| Detection |
UV 210 nm |
| Temperature |
25°C |
Discussion
This method is easy to prepare, use and reproduce a good separation of aspirin from its major hydrolysis product, salicylic acid.
Note the excellent peak shape and selectivity. Salicylic acid can be very difficult to adequately chromatograph on columns with
ordinary silica.
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Note: Aspirin, or acetylsalicylic acid (ASA) is a salicylate drug, often used as an analgesic to relieve minor aches and
pains, as an antipyretic to reduce fever, and as an anti-inflammatory medication. In countries where Aspirin is a registered
trademark owned by Bayer, the generic term is "ASA." Aspirin also has an antiplatelet or "anti-clotting" effect and is used
in long-term, low doses to prevent heart attacks, strokes and blood clot formation in people at high risk for developing
blood clots. It has also been established that low doses of aspirin may be given immediately after a heart attack to reduce
the risk of another heart attack or of the death of cardiac tissue. Aspirin was the first-discovered member of the class of
drugs known as nonsteroidal anti-inflammatory drugs (NSAIDs), not all of which are salicylates, although they all have
similar effects and most have some mechanism of action which involves non-selective inhibition of the enzyme cyclooxygenase.
Today, aspirin is one of the most widely used medications in the world, with an estimated 40,000 metric tons of it being
consumed each year.
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