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HPLC Application Sheet
Using a TYPE-C Column for Routine Reverse Phase: Benzophenone

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Note: When the percent of acetonitrile increases (from 70% in A to 80% in B) then the retention time of benzophenone (hydrophobic non ionic compound) decreases.


Method Conditions


Column Cogent Bidentate C18™, 4µm, 100A
Catalog No. 40018-75P
Dimensions 4.6 x 75mm
Mobile Phase A: 70% acetonitrile/30% DI water
B: 80% acetonitrile/20% DI water
Flow rate 0.5 mL/minute
Injection Volume  1 µL
Sample Benzophenone
0.1 mg in the mobile phase
Detection UV 254 nm


Discussion

Benzophenone, a hydrophobic non ionic compound, was selected to illustrate the excellent RP-HPLC properties of Cogent TYPE-C bonded phases. The results achieved regarding reproducibility, efficiency and peak symmetry were excellent. Each figure represents ten consecutive injections.






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