Click to Return to Home Page
Log In | Register Now

       Help


















 


 


HPLC Application Sheet
Biological Fluids: Choline & Acetylcholine

charts

PDF
Click here to view
printable Application Sheet


Method Conditions


Column Cogent Qx2™ Column for LC-MS UDC-Cholesterol, 4µm, 100A
Catalog No. 69069-Q20
Dimensions 2.1 x 20 mm
Mobile Phase  91:9 acetonitrile/DI water + 0.5% formic acid
Flow rate 0.3 mL/minute
Injection Volume 1 µL
Samples Acetylcholine (m/z 146)
Choline (m/z 104)
(5 pmol of each injected on column)
Detection Mass Spec: Atmospheric Pressure Chemical Ionization in positive mode: APCI+ Single Ion Monitoring


Discussion

The "Aqueous Normal Phase" experimental condiions shown above allow for effective separation of small polar compounds on a moderately hydrophobic stationary phase. The short and fast Qx2 format of these columns adds advantages of significantly reduced analysis time when compared with a standard column (see MicroSolv Application Note B05-1). Mass detection provides for analysis at biologically and environmentally relevant concentration levels.
Note: the model compounds used in the study are very difficult to retain on traditional hydrophobic columns.





[TOP]

Privacy Statement             Copyright © 2005, MicroSolv Technology Corporation.             Site developed by Net Ink Designs.