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3 Biogenic Amines by ANP
Biogenic amines (monoamine alkaloid, monoamine and catecholamine) analyzed reproducibly
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printable Application Sheet
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Notes: Biogenic amines are important compounds produced either by living organisms themselves (endogenous amines) or absorbed
from food (exogenous amines). Biogenic amines (such as tryptamine) if present in food or beverages and consumed in
concentration that is too high, may cause food poisoning. At the same time tryptamine, a metabolite of tryptophan is found in
trace amounts in the brains of mammals and plays a role as a neurotransmitter. Serotonin and dopamine (catecholamine) are
central nervous system neurotransmitters and are physiologically active substances; regulating the functioning of some
extrapyramidal and other brain centers.
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-15P-2 |
| Dimensions |
2.1 x 150 mm |
| Solvents |
A: DI water + 0.1% ammonium formate pH 5.9
B: 90% acetonitrile/10% DI water w/ 0.1% ammonium formate pH 6 |
| Mobile Phase |
Gradient t0 = 1.44 min
| Time |
%B |
Time |
%B |
| 0.0 |
90 |
7.0 |
70 |
| 2.5 |
90 |
7.1 |
30 |
| 6.0 |
70 |
8.0 |
30 |
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| Flow Rate |
0.4 mL/min. |
| Sample |
Mixture of 3 biogenic amines:
- Tryptamine 159.09222 m/z (M-H)-, RT = 2.23 min
- Serotonin (5-hydroxytryptamine) 175.08714 m/z (M-H)-, RT = 2.63 min
- Dopamine (3-hydroxytyramine) 152.07 m/z (M-H)-, RT = 3.51 min
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| Detection |
ESI – neg - Agilent 6210 MSD TOF mass spectrometer. |
Discussion
Chromatogram A shows separation of three biogenic amines using a Cogent Diamond Hydride™ column, buffered mobile phase for MS
and an ANP gradient (inverse). The analysis is very reproducible as shown in Figure B with excellent peak shapes. The results
presented give the strong indication on the importance of having Diamond Hydride™ or other TYPE-C Silica based HPLC columns
when analyzing basic compounds.
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