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SFC Application Sheet
Cogent Bidentate C18™ Selectivity and Retention for Carotenoids in SFC
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printable Application Sheet
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The small peaks in the chromatogram are impurities and/or degradation products in the samples. The mobile phase
modifier is methanol.
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Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-25P |
| Dimensions |
4.6 x 75mm |
| Mobile Phase |
Methanol/Carbon Dioxide (15:85 v/v) |
| Outlet Pressure |
15 mPa |
| Flow rate |
3.0 mL/minute |
| Injection Volume |
5 µL |
| Samples |
- Zeazanthin
- All trans beta-carotene
- 13 cis beta-carotene
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| Detection |
UV 440 nm |
Discussion
Cogent TYPE-C Silica™ (silica-hydride), an evolutionary material now well-established in HPLC for its
versatility and wide range of selectivities, can also be used for supercritical fluid chromatography (SFC).
In HPLC, Cogent TYPE-C columns are known to retain many polar compounds in the aqueous normal phase mode;
this application presents the separation behavior of the Cogent Bidentate C18 column for three carotenoids
(zeaxanthin, all trans beta-carotene and 13 cis beta-carotene) using the SFC technique. High efficiency and
excellent peak symmetry were obtained with this column when compared with others.
An interesting result is the relatively long retention time of zeaxanthin, which is eluted near all trans
beta-carotene. This unusual behavior displays the ability of Bidentate C18 to retain polar compounds more
strongly than typical C18 phases in SFC.
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