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Cytochrome c
From Horse and Bovine Heart

charts

Method Conditions

Column Cogent Bidentate C8 300™, 5µm, 300A
Catalog No. 40008-75P-3M
Dimensions 4.6 x 75 mm
Solvents A: DI water + 0.1% trifluoroacetic acid (TFA)
B: acetonitrile + 0.1% TFA
Gradient
Time (min.)    %B
0.0 20
16.0 40
18.0 40
18.1 20
Post time 5 min
Flow rate 0.5 mL/min.
Sample Prep 1. 1. Cytochrome c from horse heart
2. Cytochrome c from bovine heart
Detection UV 214 nm.
PDF
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printable Application Sheet

Discussion


Cytochrome c is used in the study of protein stability, folding, unfolding and molecular evolution. This protein is an efficient biological electron-transporter and is a universal catalyst of respiration.

Using the simple RP-HPLC gradient method shown in this note, it was possible to separate horse and bovine heart cytochrome c. The peaks were well separated and symmetrical. A linear detector response was observed over 2 orders of magnitude.






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