|
|
Determination of Atropine
Rugged & Fast without Paired Ions
Click here to view
printable Application Sheet
|
Note: Being potentially deadly, Atropine derives its name from Atropos, one of the three Fates who, according to Greek mythology, chose how a
person was to die. Atropine is a core medicine in the World Health Organization's "Essential Drugs List", which is a list of minimum medical
needs for a basic health care system.
|
|
Method Conditions
| Column |
Cogent UDC Cholesterol™, 4mm, 100A |
| Catalog No. |
69069-15P |
| Dimensions |
4.6 x 150 mm |
| Solvents |
A: DI water + 0.1% acetic acid + 0.005% TFA
B: Acetonitrile+ 0.1% acetic acid + 0.005% TFA
Both solutions were vacuum filtered through a 0.45µm nylon filter (MicroSolv Technology Corp.) |
| Mobile Phase |
80%A/20%B isocratic run |
| Flow rate |
1 mL/min. |
| Sample |
Prepared in 50% solution A/50% solution B, concentration 1 mg/mL.
Sample was filtered through a 0.45 µm nylon membrane HPLC filter prior to HPLC-UV injections. (MicroSolv Technology Corp.)
|
| Peak |
Atropine RT = 5.94 min |
| Detection |
UV 214 nm |
Discussion
Ion-pair chromatography (IPC) is commonly used in order to retain atropine on ordinary Type-A and Type-B reversed phase HPLC columns. Beside long
equilibration times with these columns, IPC often suffers from poor robustness. The aim of this study was to develop a robust and simple HPLC method
for testing of atropine. Using a Cogent UDC-Cholesterol™ column and an isocratic elution with flow rate 1 mL/min gave symmetrical peak for this
tropane alkaloid.
The method presented is simple, accurate and reproducible. The sensitivity is sufficient for the proper determination of atropine in plasma after
intravenous administration of the drug to hospitalized patients. This method is also useful for testing for drug poisoning and for stability testing of
atropine solutions during manufacturing. The linear range of detection for atropine was around 5.0 µg/mL with a limit of quantification (LOQ) 10.0
µg/mL.
|
