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Domoic Acid in Seafood Samples
Domoic Acid (DA) From Seafood Without Derivatization
| A – | Extract from mussels (low concentration) |
| B – | Extract from mussels (high concentration) |
| C – | Fish extract |
| D – | Plankton high and low concentration of domoic acid |
| E – | Standard of domoic acid |
| F – | 5 Overlaid injections of plankton extract sample |
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printable Application Sheet
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Notes: Data was presented at the American Society of Pharmacognosy Annual Meeting and Exhibition, July 30 – August 3, 2011, San Diego,
California.
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-15P-2 |
| Dimensions |
2.1 x 150 mm |
| Solvents |
| A: | 50% MeOH/ 50% DI H2O/ 0.1% formic acid |
| B: | Acetonitrile/ 0.1% formic acid |
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| Gradient |
| time (min.) |
%B |
time (min.) |
%B |
| 0 |
95 |
8 |
30 |
| 7 |
30 |
10 |
95 |
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| Post Time |
5 min |
| Injection Vol. |
1 microL |
| Flow rate |
0.4 mL/min. |
| Detection |
ESI - POS - Agilent 6210 MSD TOF MS |
| Samples |
Samples from Dr. R.Kudela, Univ. of Calif., Santa Cruz,
Dept. of Marine Sciences. Samples were injected as received. |
| Peak |
Domoic acid 312.1442 m/z (M+H)+ |
| t0 |
0.9 min |
Discussion
Several seafood and plankton samples were tested for domoic acid (DA) which, as a highly polar compound, is poorly retained on most reversed phase
columns. This developed method based on HPLC and MS detection does not require derivatization of DA. The concentration of this potent toxin varied
quite substantially between the samples (from 40 ng/mL in plankton to 3700 ng/mL in mussels). Despite different sample matrices, it was possible to
detect and analyze domoic acid in a wide range of concentrations.
The results were very repeatable (see 5 overlaid injections in Figure F). The detection limit depends on the instrument used. The method can be used
by regulatory agencies responsible for monitoring the occurrence of toxins such as the analysis of domoic acid in seafood samples.
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