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Drug Metabolite
4’-aminoacetophenone
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Note: 4’-aminoacetohenone (arylamine) is one of the metabolites of 1-(4-acetylphenyl)-3,3-dimethyltriazene, which is an
antitumour triazene. The presented method could be useful in analysis of the metabolites of this class of antitumour agents
in body fluids (plasma or urine) during or after chemotherapy.
4’-acetaminophenone is also frequently used as an internal standard in analysis of Mitomycin C.
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Method Conditions
| Column |
Cogent Bidentate™, 4µm, 100A |
| Catalog No. |
40018-75P |
| Dimensions |
4.6 x 75 mm |
| Mobile Phase |
80% A/20% B
Solvent A: 100% DI water + 0.2% acetic acid
Solvent B: 100% acetonitrile + 0.2% acetic acid |
| Flow rate |
1.0 mL/minute |
| Injection Volume |
2 µL |
| Sample |
1mg of the compound dissolved in 1 mL of 50% A/50% B solution. Sample for injection diluted 1:15 with 100% A.
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| Detection |
UV 324 nm |
Discussion
The use of a Cogent Bidentate™ C18 column in RP-HPLC mode was investigated using 4’-acetaminophenone as a model compound. The
short column gave adequate retention and sensitivity for the analyzed compound.
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