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Very Sensitive Method for
EDTA: ethylenediaminetetraacetic acid
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printable Application Sheet
| Note: EDTA is a synthetic metal complexing reagent that is used in a wide variety of industrial applications. Used as a
preservative, it has very low biodegradability thus remains in the environment for long periods of time. Found in sewer
water, freshwater and ground water, it re-solubilizes precipitated toxic metals back into solution where they can be
ingested by plants and animals. |
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Method Conditions
| Column |
Cogent HPS C8™, 5µm, 120A |
| Catalog No. |
75008-15P |
| Dimensions |
4.6 x 150mm |
| Mobile Phase |
2% Acetonitrile, 98% DI Water 0.1% Acetic Acid
pH 3.5, plus 2g/L Tetrabutylammonium sulfate |
| Flow rate |
2.0 mL/minute |
| Peaks |
1. Water (solvent front)
2. EDTA Fe3+ |
| Injection Volume |
20 µL |
| Detection |
UV 258 nm |
| Temperature |
40°C |
| LOQ |
0.2µg/mL |
Discussion
EDTA does not have a significant chromophore, so to achieve UV detection this method uses a pre column reaction of a solution of
ferric chloride with the sample. The resulting EDTA/Fe3+ has significant UV absorbance making this a very sensitive method.
Excellent peak shapes and selectivity make the Cogent HPS C8™ a great choice for this method.
EDTA is extremely difficult to analyze by itself however in its complexed form, it chromatographs well from matrices such as river
sediment and other solutions.
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