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Fioricet®/Esgic®
Gradient separation of Acetaminophen, Caffeine, and Butalbital
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printable Application Sheet
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Note: Fioricet®/Esgic® is a combination of acetaminophen, caffeine, and butalbital. It is used to treat tension headaches, muscle
contraction headaches, and migraines.
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Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-15P |
| Dimensions |
4.6 x 150 mm |
| Solvents |
A: DI water + 0.1% formic acid
B: 97% acetonitrile/ 3% DI water/ 0.1% formic acid |
| Gradient |
| time (min.) |
%B |
| 0 |
10 |
| 6 |
70 |
| 7 |
10 |
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| Injection Volume |
10 microL |
| Flow rate |
0.8 mL/min |
| Temperature |
35°C |
| Detection |
UV 240 nm |
| Sample |
Stock Solution: Fioricet® tablet was ground, added to 10 mL volumetric flask and diluted with 50/50 Solvent A/Solvent B
mixture. The flask was vortexed 5 min and a portion of the solution was filtered with a 0.45 µm nylon syringe filter.
Working Solution: 10 µL of the stock was diluted with 990 µL of 50/50 Solvent A/Solvent B mixture. |
| Peak |
1. Acetaminophen
2. Caffeine
3. Butalbital |
| t0 |
2.2 min |
Discussion
The method shown uses a Bidentate C18™ column with a simple reversed phase gradient to separate the three components of a Fioricet® tablet.
Figure A shows the five-run overlay obtained from the gradient. Figure B shows a “zoomed in view” so that the butalbital peak can be seen clearly.
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