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Glyphosate: Herbicide
ANP Retention of an Extremely Polar Compound
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printable Application Sheet
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Notes: Glyphosate is a nonselective herbicide and is used for the control of a wide range of weeds. It is strongly retained
on soil components and due to its long half life and solubility in water can be detected long after application or far away
from the application site.
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-15P-2 |
| Dimensions |
2.1 x 150 mm |
| Solvents |
A: DI water + 5 mM ammonium acetate
B: 90% acetonitrile/10% DI water/10 mM ammonium acetate |
| Gradient |
0.0 min – 80.0%B
1 .0 min 80%B
1.1 min to 5%B
5.0 min 5%B
6.0 min to 80%B |
| Post time |
5 min |
| Flow rate |
0.5 mL/min. |
| Sample Prep |
Glyphosate: 168.0067m/z (M - H)-
Figure A: injection #1, RT = 3.365 min
Figure B: injection #5, RT = 3.366 min
Sample stock solution was purchased from Sigma (1000 mcg/mL). Sample
for analysis was made by diluting the stock 1:100 in 30:70 solution A and B.
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| Detection |
ESI – neg - Agilent 6210 MSD TOF mass spectrometer. |
Discussion
A reliable method for the determination of glyphosate is presented. Analysis was performed using a Cogent Diamond Hydride™ HPLC
column which provides very reproducible retention and fast equilibration even when a gradient analysis is used. The use of LC-MS
detection allows avoiding time consuming derivatization of this compound which is lacking a chromophore for UV detection. The method
shown, with ANP retention avoids derivatization, which is required when an ordinary C18 column is used.
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