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CapLC Application Sheet
Separate of Hormones with Cogent Bidentate C18™ Capillary LC Column in Reverse Phase
Shorter Analysis Time
Method Conditions
| Column |
Cogent Bidentate C18 capillary LC Column, 4µm, 100A |
| Catalog No. |
40018-A-50 |
| Dimensions |
50 µm i.d. x 40 cm packed
(50 cm total length) |
| Mobile Phase |
70:30 acetonitrile/DI water + 0.1% formic acid |
| Flow rate |
100 nL/minute |
| Injection Volume |
10 nL |
| Sample |
1. Prednisolone
2. Corticosterone
3. Norgestel
4. Progesterone
100 mg/mL of each in acetonitrile + DI water
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| Detection |
UV 254 nm |
| Instrument |
Trisep™ - 2100 EPLC system, Unimicro Tech. Inc. |
Discussion
The chromatogram presented was done in Capillary LC and shows the separation of mixture of four steroids. The peak
shape of each compound is excellent (As < 1.1 for all solutes) using an isocratic mobile phase. The Signal to Noise
ratio is excellent, due to a high order digital filter used in the detector. Small peaks of impurities and
degradation products of hormones tested are visible. The instrument is a valuable addition to any analytical
laboratory.
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