| Column |
Fig. A: MTC Phenyl Hydride™, 4µm, 100A
Fig. B: Type B silica-based Cyano, 5µm 100A |
| Catalog No. |
Fig. A: 69020-7.5P Fig. B: N/A |
| Dimensions |
Fig. A: 4.6 x 75 mm Fig. B: 4.6 x 250 mm |
| Mobile Phase |
Fig. A: A - DI water/ 0.1% formic acid
& nbsp; B - 97% Acetonitrile/ 3% DI water/ 0.1% formic acid
Fig. B: 60% DI water/ 40% Acetonitrile/ 0.05% phosphoric acid |
| Gradient |
Fig. A:
| time (min.) |
%B |
| 0 |
20 |
| 6 |
50 |
| 7 |
20 |
|
| Temperature |
Fig. A: 35° C Fig. B: ambient |
| Flow rate |
Fig. A: 1.0 mL/min Fig. B: 1.5 mL/min |
| Injection Volume |
Fig. A: 2µL Fig. B: 100µL |
| Sample |
Mix of levothyroxine and liothyronine standards
Stock Solutions: 0.4 mg levothyroxine or liothyronine dissolved with 1 mL 10 mM NaOH in 50:50 DI water: methanol. Working
Solution (Fig. A): Aliquots of stock solutions were mixed and diluted with 50:50 A:B to obtain concentrations of 40 mg/L and 4
mg/L for levothyroxine and liothyronine respectively. Working Solution (Fig. B): Aliquots of stock solutions were mixed and
diluted with the mobile phase to obtain concentrations of 10 mg/L and 0.2 mg/L for levothyroxine and liothyronine
respectively. |
| Peaks |
1. liothyronine sodium, 2. levothyroxine sodium |
