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HPLC Application Sheet
Normal Phase Made Easier: Drying Solvents in the Mobile Phase not Necessary

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Method Conditions


Column Cogent Bidentate C18™, 4µm, 100A
Catalog No. 40018-75P
Dimensions 4.6 x 75 mm
Mobile Phase 95:5 Hexane/Ethyl Acetate
Flow rate 1.0 mL/minute
Injection Volume  1 µL
Samples 1 mg/mL of proprietary compounts were dissolved in the mobile phase
Peaks
  1. Phenolic compound with an aldehyde
  2. Parent phenolic compound
  3. Phenolic compound with a ketone
  4. Phenolic compound with an acid group
Detection Photo Diode Array (UV)


Discussion

This Cogent™ Bidentate C18 column was used to quickly separate four proprietary phenolic compounds (precursors for a catalyst) under Normal Phase conditions. The separation shown is extremely reproducible (%RSD 0.2) and the solvents do not need to be dried before use as with other normal phase columns, saving time and lab resources.

Notes:
  1. This method can also be used with Atmospheric Pressure Chemical Ionization in the positive or negative mode: APCI+, APCI-
  2. The column used for this application was previously used under Reversed Phase condition. Switching the column to Normal Phase mode required flushing 100% methanol for 10 minutes followed by 100% Methylene Chloride for 10 minutes. This process can be repeated over and over without damage to the column and can go back and forth between modes.







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