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Nucleotide Bases: Orthogonal Method
Excellent Peak Shape, Resolution, MS Friendly
Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-75P |
| Dimensions |
4.6 x 75 mm |
| Mobile Phase |
DI Water + 0.1% Acetic Acid |
| Flow rate |
1.0 mL/min. |
| Sample |
1. Uracil
2. Guanine
3. Thymine
4. Adenine
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| Injection Volume |
2.5 µL |
| Detection |
UV 254 nm |
| Temp |
25° C |
Discussion
This method is easy to prepare, use and reproduce. Note Excellent separation under 100% aqueous conditions with alternate selectivity.
These bases are difficult to retain on columns with ordinary silica that contain significant amounts of silanols.
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Notes: Nucleobases (or Nucleotide bases) are the parts of DNA and RNA that may be involved in pairing . The main bases are
cytosine, guanine, adenine (DNA and RNA), thymine (DNA) and uracil (RNA), abbreviated as C, G, A, T, and U, respectively.
They are usually simply called bases in genetics. Because A, G, C, and T appear in the DNA, these molecules are called
DNA-bases; A, G, C, and U are called RNA-bases.
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