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HPLC Application Sheet
Polycyclic Aromatic Hydrocarbons (PAH): A Reverse Phase Separation

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Note: Cogent Bidentate C18 with Selectivty3™ operates in three modes of chromatography (reversed phase, aqueous normal phase and organic normal phase). Separation of PAHs illustrates its excellent capabilities in the reversed phase mode.

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Method Conditions


Column Cogent Bidentate C18, 4µm, 100A
Catalog No. 40018-75P
Dimensions 4.6 x 75mm
Mobile Phase Acetonitrile/DI Water 70:30
Flow rate 0.5 mL/minute
Injection Volume  1 µL
Samples
  • Naphthalene
  • Phenanthrene
  • Fluorene
  • Pyrene
  • Impurities or decomposition products - 1mg of
    each sample was dissolved in 1 mL of the mobile
    phase
    		•
    Detection UV at 254 nm


    Discussion

    PAH (Polycyclic Aromatic Hydrocarbons) determination in several matrices (soil, food, air, body fluids etc.) is a topic of interest for routine quality control or screening analyses. Cogent Bidentate C18™ columns offer good resolution, reproducible retention time and peak shape for PAH compounds. In addition to the main components, small peaks due to impurities or decomposition products are well resolved.






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