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Glycoprotein:
Human Plasma
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printable Application Sheet
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Notes: Many genetic disorders can be diagnosed by analysis of serum glycoproteins. The analysis has a potential of finding new
biomarkers. In addition the concentration of glycoproteins in plasma can vary considerably as a response to various stressful
events, for example surgery.
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Method Conditions
| Column |
Cogent Bidentate C8 300™, 5µm, 300A |
| Catalog No. |
40008-75P-3M |
| Dimensions |
4.6 x 75 mm |
| Solvents |
A: DI water + 0.1% trifluoroacetic acid (TFA)
B: acetonitrile + 0.1% TFA |
| Gradient |
| Time (min) |
%B |
| 0.0 |
15 |
| 10.0 |
100 |
| 20.0 |
100 |
| 21.0 |
15 |
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| Post time |
5 min |
| Flow rate |
0.5 mL/min. |
| Sample Peak |
a2-hs-glycoprotein, human plasma, lot #074K16644. 1 mg/mL in DI water.
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| Detection |
UV 214 nm |
| Injection |
1 microL |
Discussion
Using the method in this note, a typical chromatogram of a solution of human plasma glycoprotein is obtained. The elution times for
this glycoprotein were between 7.25 and 7.35 min, with any day-to-day variations occurring within these times. Confusion of the
a2-hs-glycoprotein with other plasma proteins is minimal since the major plasma protein (HSA – human serum albumin) is usually removed
before HPLC analysis in a precipitation step.
The method presented is generic and it is suitable for analysis of a2-hs-glycoprotein and other glycoproteins in physiological fluids.
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