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Standard Protein Mixture
High Resolution & Efficiency
Method Conditions
| Column |
Cogent Bidentate C8 300™, 5µm, 300A |
| Catalog No. |
40008-75P-3M |
| Dimensions |
4.6 x 75 mm |
| Solvents |
A: DI water + 0.1% trifluoroacetic acid (TFA)
B: acetonitrile + 0.1% TFA |
| Gradient |
| Time (min) |
%B |
| 0.0 |
20 |
| 10.0 |
80 |
| 12.0 |
80 |
| 13.0 |
20 |
|
| Post time |
5 min |
| Flow rate |
0.5 mL/min. |
| Sample Peak |
1. Ribonuclease A
2. Cytochrome c
3. Holo-transferrin
4. Apomyoglobin
|
| Detection |
UV 214 nm |
Discussion
HPLC is the premier technique for the analysis and purification of a wide range of protein and peptides and is used for analytical and
preparative applications. It is extremely versatile for the isolation of proteins from a variety of synthetic or biological sources.
The method shown in this note for a protein mixture offers excellent resolution which can be achieved over a wide range of
chromatographic conditions. The gradient separation shown is extremely reproducible (%RSD around 1) over a long period of time and the
peaks are fully resolved and symmetrical.
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