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Succinic Acid: Rapid & Cost Effective
Isocratic or Gradient
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printable Application Sheet
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Notes: There is large interest in the production of this organic acid because it is a key compound in producing more than 30
commercially important products including Pharmaceuticals, foods, dyes, perfumes, lacquers, metal treatment, water cooling
and many other uses. An intermediate of tricarboxylic acid cycle, succinic acid is produced and accumulated by anaerobic
microorganisms.
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-15P-2 |
| Dimensions |
2.1 x 150 mm |
| Solvents |
A: 50% Methanol/50% DI water + 10mM ammonium acetate
B: 90% Acetonitrile/10% DI water + 10mM ammonium acetate |
| Gradient |
RT succinic acid = 5.33 min
0 min 100% B
0–5 min 50.0% B
5-7 min 50% B
7-9 min 100% B
10 min 100% B
Post time: 5 min. |
| Flow rate |
0.4 mL/min. |
| Sample |
Succinic acid 117 m/z (M - H)- 100 ng/mL prepared in 50%A/50%B and diluted 1:10 before analysis
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| Detection |
ESI – neg - Agilent 6210 MSD TOF mass spectrometer. |
Discussion
The analysis of succinic acid is mostly performed by gas chromatography (GC), enzymatic assays, ion-exclusion chromatography (IEC) or by reversed phase HPLC. However, these techniques are time consuming and analysis time is very often longer than 1 hour.
In this application note we are reporting a rapid, cost effective screening method for the detection of this important organic acid. This method can be utilized to screen a large number of microbes producing succinic acid in a very short time (under 10 minutes). This proposed method was found to be an attractive choice for the analysis of this compound in drug dissolution media, in which is it used as a pH adjuster.
Note:
When an isocratic runs were used, at 95% B succinic acid was indefinitely retained on DH column, so linear gradient conditions were used for analysis of this important acid. All Type-C cogent columns equilibrate very rapidly to initial conditions when gradient analysis is used.
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