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HPLC Application Sheet
Norgestrel, ETES & Impurity (4) - Cogent UDC-Cholesterol™

Chromatogram A
Chromatogram B
Chromatogram C

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Method Conditions


Mobile Phase   
  Chromatogram A:  35% Aqueous (0.1%TFA)
65% MeOH
Chromatogram B:  42% Aqueous (0.1%TFA)
58% MeOH
Chromatogram C:  45% Aqueous (0.1%TFA)
55% MeOH
Column Cogent UDC-Cholesterol™
Catalog No. 69069-75R
Dimensions 75 x 4.6mm id
Flow Rate 1.0ml/min
Detection UV, 240nm
Temperature 0°C


Discussion of Chromatograms

Finding trace impurities is a key element of pharmaceutical research. No trace impurities were found in ETES when using:
  • both ACN and MeOH with a variety of C18 columns
  • only ACN at various percentages on a UDC-Cholesterol™ column
  • MeOH on the UDC-Cholesterol™ column at temperatures above 15°C
At 0°C on the UDC-Cholesterol™ column as the percentage of MeOH is sequentially reduced from 65% to 55%, it becomes obvious that a previously unresolved component is present.
Implications, Advantages & Uses
Using the unique, shape recognition, selectivity mechanism (MeOH) on the UDC-Cholesterol™ column at low temperatures allowed resolution of a previously undetected impurity in a test steroid.





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