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HPLC Application Sheet
Glyburide & Metformin Mixture - Cogent UDC-Cholesterol™
Retention Map: Useful tool, for quickly developing methods in HPLC: Here, Phenol exhibits a typical reverse phase plot (ARP) while both Uracil and Pyridine are showing normal phase characteristics in aqueous conditions (ANP). Discussion of Chromatograms Chromatograms A & B Peak 1 (Metformin) eluted with longer retention time as the concentration of Acetonitrile was increased. During the same run, Peak 2 (Glyburide) elutes with shorter retention time as concentrations of Acetonitrile are increased. Implications and Advantages Metformin is a very polar pharmaceutical compound which, chromatographically, is very similar to Pyridine (see Retention Map above). Glyburide, a neutral pharmaceutical ingredient is often formulated with Metformin, which is so polar that it elutes on the solvent front on other C18, Cyano, Amino, Phenyl and F5 columns. To quantify these compounds, two runs with different methods were previously necessary. Uses By using one UDC-Cholesterol™ HPLC Column, you can isocratically achieve separation and get confirmation of your peaks with the column’s orthogonal selectivity. Simply modify the mobile phase as shown here to save time and lab resources. |
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