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Method Conditions

Column: Cogent HPS™ C18, 150mm x 4.6mm
Type of Hardware: Super-Link System™
Catalog Number: 75018-15 or 75018-15R
Flow Rate: 1.0mL/min
Temperature: Ambient
Detection: 254nm
Injection: 10µL
Mobile Phase: 90:10 Methanol:Water
Test Mixture: Uracil, Dimethyl Phthalate, Toluene, Biphenyl, Phenanthrene.

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Peak	tR	k'	Symmetry	USP Tailing	N/M
1: Uracil	1.73	0.00	1.78	1.11	62,873
2: Dimethyl Phthalate	2.04	0.46	0.17	1.12	72,780
3: Toluene	2.95	0.70	0.98	0.99	94,307
4: Biphenyl	3.75	1.16	0.94	0.96	93,507
5: Phenanthrene	4.54	1.62	0.92	0.94	91,640

Discussion of Chromatogram:
This is an industry standard method to show the peak shape and efficiency of any column for neutral compounds. The peak shape and efficiency of Phenanthrene is an indication that this column is an excellent choice for many neutral compounds. With optimizing the mobile phase/buffer, such as using Methanol, Acetonitrile orTHF, the peak shape and efficiency could easily be further improved.