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Method Conditions

Column: Cogent HPS™ C18, 150mm x 4.6mm
Type of Hardware: Super-Link System™
Catalog Number: 75018-15 or 75018-15R
Flow Rate: 1.0mL/min
Temperature: Ambient
Detection: 215nm
Injection: 10µL
Mobile Phase: 80:20 Methanol:25mM KH2PO4
pH: 6.0
Test Mixture: Uracil, Nortriptyline, Toluene, Imipramine, Amitryptiline

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Peak	tR	k'	Symmetry	USP Tailing	N/M
1: Uracil	1.73	0.00			50,580
2: Nortriptyline	3.94	1.28			45,567
3: Toluene	4.26	1.47			89,360
4: Imipramine	7.68	3.45			65,947
5: Amitryptiline	9.39	4.44	1.65	1.33	69,327

Discussion of Chromatogram:
This is an industry standard method to show the peak shape and efficiency of any column for hard to separate antidepressants. The high efficiency of this column as shown in the Amitryptiline peak is an indication that this column is an excellent choice for many difficult to separate basic drugs. With optimization of buffer and organic solvents the peak shape and efficiency could easily be further improved and peaks two and three could be base line resolved.