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Method Conditions

Column: Cogent HPS™ C18, 150mm x 4.6mm
Type of Hardware: Super-Link System™
Catalog Number: 75018-15 or 75018-15R
Flow Rate: 1.0mL/min
Temperature: Ambient
Detection: 215nm
Injection: 10µL
Mobile Phase: 35:65 ACN:Water/0.1% TFA
Test Mixture: Uracil, 4-Hyroxy BZA, Acetylsalicylic Acid, Benzoic Acid, 2-Hydroxy BZA, Ethyl Paraben

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Peak	tR	k'	Symmetry	USP Tailing	N/M
1: Uracil	1.56	0.00	1.26	1.17	67,213
2: 4-Hyroxy BZA	2.41	0.54	1.10	1.05	43,600
3: Acetylsalicylic Acid	4.00	1.56	0.99	0.98	71,193
4: Benzoic Acid	4.52	1.89	0.96	0.97	78,900
5: 2-Hyroxy BZA	5.91	2.78	0.98	0.97	82,607
6: Ethyl Paraben	7.73	3.94	0.92	0.94	82,713

Discussion of Chromatogram:
This is an industry standard method to show the peak shape and efficiency of any column for Acidic Compounds. The high efficiency and excellent peak shapes indicates that this column is an excellent choice for acids. Peak 5, 2-Hydroxy Benzoic Acid would tail badly with older technology columns (click Here to See Comparative Chromatograms) that have a higher metal content in the stationary phase.