| |
HPLC Application Sheet #126
Market Leader Column Separating Basic Compounds
< Back to the previous Application Sheet
Method Conditions:
Column: Brand Y (BDS) C18, 150mm x 4.6mm
Type of Hardware: Compression Fittings
Flow Rate: 1.0mL/min
Temperature: Ambient
Detection: 254nm
Injection: 10µL
Mobile Phase: 60:40 Methanol:Water
Test Mixture: Uracil/Pyridine/Phenol
Peak
|
tR
|
k'
|
Symmetry
|
USP Tailing
|
N/M
|
1: Uracil
|
1.67
|
0.00
|
1.41
|
1.24
|
43,360
|
2: Phenol
|
2.43
|
0.46
|
1.32
|
1.20
|
62,113
|
3: Pyridine
|
5.22
|
2.13
|
6.05
|
3.90
|
2,793
|
|
|
