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Care of Standard Cogent HPLC Columns
How to Store Cogent HPLC Columns
Long Term Storage:
- Rinse the column with 8 column volumes with pure solvent that you use as your mobile phase. It is important to
use non-buffered mobile phase.
- Prepare the column for storage by purging it with 8 column volumes of 100% HPLC Grade, filtered Isopropyl
Alcohol (IPA).
- Purge the column with 80% IPA and 20% water with 8 column volumes.
- Cap both ends of the column with the provided protective end caps or with Super-Link End Fittings and capping
them with column plugs provided.
- Return column to the box it came in for safe-keeping.
- Store column at room temperature.
- Never let the column dry out.
- Never store the column in buffers.
- Never store the column in liquid that is more than 30% water.
Overnight:
- Rinse the column with 8 column volumes with pure solvent that you use as your mobile phase. It is important to
use non-buffered mobile phase.
- Cap both ends of the column with the provided protective end caps or with Super-Link End Fittings and capping
them with column plugs provided.
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