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HPLC Application
Pyridine/Phenol
(Silanol/Metals Activity Test)

Pyridine Test (Silanol/Metals Activity):
Mobile Phase:	60:40 Methanol/Water
Flow Rate:	1.0 ml/min
Detection:	254nm
Injection:	10µl
Temp:	Ambient

Test Results for Pyridine Test
Click on any analyte to view its structure.

Column Name (Listed Alphabetically)	Analytes	Polarity k' Pyr/Phnol	Plates/Meter	Symmetry	USP Tailing
Cogent HPS C18 Catalog No.: 75018-15R Click Here to View Product Literature	Uracil Pyridine Phenol	0.58	47,420 32,113 70,220	1.21 3.26 1.03	1.12 2.54 0.99
Beta Basic C8	Uracil Pyridine Phenol	0.54	52,487 35,693 68,513	1.31 1.85 1.15	1.21 1.60 1.09
Hypersil BDS C18 Note change in elution order	Uracil Phenol Pyridine	4.68	43,360 62,113 2,793	1.41 1.32 6.05	1.24 1.20 3.90
Inertsil ODS--3	Uracil Pyridine Phenol	0.51	33,580 41,287 51,873	1.30 1.25 1.16	1.19 1.19 1.09
Kromasil C18	Uracil Pyridine Phenol	0.57	36,460 18,920 62,413	1.52 3.86 1.39	1.33 2.90 1.25
Luna C18 (2)	Uracil Pyridine Phenol	0.49	43,620 36,840 71,220	1.38 2.26 1.27	1.24 1.90 1.17
Selectosil C18 Note change in elution order	Uracil Phenol Pyridine	4.92	6,752 8,514 263	1.24 1.08 8.90	1.14 1.05 4.37
Spherisorb ODS 2 Note change in elution order	Uracil Phenol Pyridine	1.55	38,507 62,333 713	1.16 1.24 2.88	1.13 1.17 2.59
YMC Basic C8	Uracil Pyridine Phenol	0.61	53,293 35,380 70,267	1.49 2.07 1.38	1.32 1.63 1.27
Zorbax Extend C18	Uracil Pyridine Phenol	0.56	41,313 34,420 64,087	1.44 2.10 1.25	1.29 1.76 1.15

Chromatograms





Cogent and HPS are trademarks of MicroSolv Technology Corporation, Inertsil ODS-3 is a trademark of GL Sciences, Kromasil is a trademark of Akzo Nobel, Zorbax Extend is a trademark of Agilent Technologies, Inc., Spherisorb ODS2 and YMC Basic are trademarks of Waters Corporation, Beta Basic C8 and Hypersil BDS C18 are trademarks of Thermo Corporation, Selectosil C18 and Luna C18 are trademarks of Phenomenex Corporation.