For Best Results with Jordi™ Brand Columns

• TIPS FOR BEST RESULTS GPC/SEC COLUMNS
• TIPS FOR BEST RESULTS WITH REVERSE PHASE COLUMNS
• AVOIDING TAILING AND/OR ADSORPTION PHENOMENA ON JORDI™ BRAND COLUMNS

TIPS FOR BEST RESULTS GPC/SEC COLUMNS:

1. Flow rate for your column should be between 0.5mL and 2.0mL per minute for maximum life and best results. A flow rate of 1.5mL per minute is most likely to be optimal.
2. Using Jordi™ columns in TCB at 140-150°C, it is important to purge the columns at room temperature at 0.2mL per minute overnight with TCB and then ramp up to full temperature over 300 minutes.
3. Keep your frits clean and clear; if you notice your calibration change after significant use, clean the frits of your column (particularly on the inlet side). The original frit is 2µm and if it is clogged, this will contribute to the shearing of high MW polymers. It is important that frits remain unclogged for good results.
4. If your column needs to be regenerated, it is possible to return it to MicroSolv for re-packing. Please inquire for pricing.
5. For use of special solvents such as DMSO and Water, Methanol, Acetone, it is best to contact our technical service department for special instructions.
6. For any solvent change involving miscible solvents it is best to first purge the column with the new solvent at 0.2mL per minute overnight. Immiscible solvents require an intermediary solvent that both the initial and final solvents are miscible in. For suggestions, please contact us.
7. For Mixed-Bed columns, or 104 and 105A columns, Never exceed 2,000 psi. Excessive pressures could crush the gels. For other sizes, pressures up to 6,000 psi will not cause any damage.

TIPS FOR BEST RESULTS WITH REVERSE PHASE COLUMNS

1. Run reverse phase columns at the flow rates in the chart below:

   Column ID (mm)	Flow Rate mL/minute
   4.6	0.5 – 1.5
   10	1.0 – 2.0
   22	3.0 – 6.0

2. Do not worry about high back pressures. The Jordi™ reverse columns are packed at 8,000 psi and can run for months in the 3,000 – 5,000 range without damage to them.
   Due to the limitations of the 1” hardware, do not run the 1” diameter columns over 3,000 psi.
3. Keep your frits clean and clear; if you notice your calibration change after significant use, clean the frits of your column (particularly on the inlet side). The original frit is 2µm and if it is clogged, this will contribute to the shearing of high MW polymers. It is important that frits remain unclogged for good results.
4. Whenever possible, keep at least 10% organic in your mobile phase composition. Due to very hydrophobic nature of the Jordi™ gels, they will not ”wet” in water. If you need to use pure water/buffer as your mobile phase, the gels will shrink slightly (about 5%) and result in loss of efficiency.

AVOIDING TAILING AND/OR ADSORPTION PHENOMENA WITH JORDI™ COLUMNS

The Jordi™ DVB HPLC products are made from cross linked polymers of divinylbenzene. Due to the large quantity of aromatic rings in the backbone of the stationary phase, a very unique response to certain types of samples will result.

If samples of interest contain aromatic rings or atoms such as oxygen or nitrogen with unshared electron pairs, they have the potential to be strongly retained on the column. Using a “competing” electron rich solvent as the mobile phase will sharpen your peaks and allow for elution of these samples.

Using these solvents will “adjust” the surface chemistry of the stationary phase by coordinating with the aromatic rings creating a less electron dense surface chemistry.

PEAK MODIFIERS

In some cases, it may be possible to use Sodium Acetate to modify the peaks and retention intensity. Some suggested mobile phase additives are TEA (Triethylamine), n-Butylamine, Sodium Acetate, Glycerol, 2-Propanol or other similarly hydrophilic hydroxylated solvents.

It is best to use 50:50 mixture of Methanol: Acetonitrile as a strong solvent but you can also use 0.5 – 2.0% TEA or Ethylene Glycol or 0.01M Sodium Acetate as peak modifiers for sharpness when needed.