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The Problem

Chromatogram A
Cogent HPS™ C18

Compounds 2 & 4 and 3 & 6 Co-Elute
 		Test Mixture Compound Number:
  1. Phenol
  2. Dimethyl Phthalate
  3. Toluene
  4. Nortriptyline
  5. Imipramine
  6. Amitryptiline

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 		Chromatogram B
Cogent HPS™ Cyano

Compounds 1, 2 & 3 and 4, 5 & 6 Co-Elute

Above are two isocratic separations on two Cogent Mini-Scout HPLC Columns. Each column is a highly efficient, state of the art column. Neither of these columns are able to resolve all of the compounds in the test mixture.

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