MicroSolv Technology Corporation

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Hydrophobic and Silanophilic Interaction Tests
for Cogent™ TYPE-C™ Silicon-Hydride
HPLC Columns
The “Neue Test”

Actual Chromatograms: Silanophilic Test
Phenomenex Luna C18 (2)™
4µm, 4.6 x150mm
Catalog No.: 00F-4252-EO

Method Conditions:
Mobile Phase: 35:65, 20mM phosphate buffer, pH 7/Methanol
Flow Rate: 1.0ml/min
Injection Volume: 1µl
Detection: UV 214nm

Sample:

Dissolved in Methanol
Amitriptyline Hydrochloride 100 mg/L

Assymmetry Parameter (As): 1.730

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