Aqueous reverse phase=ARP
Aqueous normal phase=ANP
Organic normal phase=ONP

To avoid confusion, we have defined ANP and ONP as different from one another, but they are really different ends of the same continuum. Thus mobile phase solvents hexane/ethyl acetate and 60+% acetonitrile/buffered water (for ionic compounds at appropriate pH) are both normal phase separations on TYPE-C™ products.

It is the uniqueness of TYPE-C™ phases that allows highly polar eluents such as acetonitrile/buffered water to be utilized in the normal phase mode that offers chromatographers unique separation solutions.

This presentation will also show you how to utilize MicroSolv’s “Retention Maps”, taken at three different pH levels, for target Acid, Base & Neutral compounds of known Octanol-Water Partition Coefficients and to be able to predict the retention behavior of your compound on our Type B and TYPE-C™ phases to assist your rapid method development.

Finally, in this presentation you will see that by just changing the acetonitrile percentage of the mobile phase, it is possible to baseline resolve and quantify, in a 3 minute isocratic run with Cogent UDC Cholesterol™ columns, the pharmaceutical formulation of Metformin and Glyburide.

We also present the novel twist of being able to elute either the most or least polar compound first, or make them co-elute, or have either on the solvent front and the other infinitely retained.

We have challenged competitors to repeat this feat - none have yet. Now we ask: CAN YOUR HPLC COLUMN DO THIS?