MicroSolv Technology Corporation

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Can Your HPLC Column Do This?
Comparison of type B and TYPE-C Silica™ Using Aqueous Mobile Phase – Page 3

Few chromatographers have experience with Aqueous Reverse Phase on irregular, type-A or type-B un-bonded silica. What typically happens is there is little or no retention or compounds that are retained after the solvent front, rapidly become very broad, such that a peak with 3 minute retention might have a minute or more peak width. This is generally unacceptable.

On this page, in the two chromatograms, you can see silica from the same production batch. The first chromatogram remains as the initial type B, spherical, low trace metal bare silica but, the second chromatogram shows the enormous reduction in phase polarity that conversion to TYPE-C- the evolutionary silica- achieves.

This reduction of polarity is caused by conversion of most of the silanol (Si-OH) sites to silicon-hydride (Si-H).

To visualize the impact of this, think of a standard C18 alkane, very non-polar, insoluble in water, now consider what if converted all Hydrogen to OH...?

This is the similar impact that our patented process is having on silica.

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