Can Your HPLC Column Do This?
The Evolution of HPLC Silica Particles – Page 4
The HP ( High Performance ) of HPLC began with the first 10 um irregular
silica based reverse phases, but true reproducibility did not start until
spherical particles where introduced.
These early spherical phases then evolved to type B spherical phases, which
had the added benefit of low trace metals in the silica, thereby reducing
the impact of silanols ( through reduced electron withdrawal enhancement of
the silanol activity ) on peak tailing with certain compounds.
TYPE-C™ takes this evolution to reduce silanol activity one stage further.
With a bonded and fully end-capped type B phase approximately 30%-50% of the
available silanols ( approximately 20% of the surface ) are still non end capped.
With TYPE-C™, hydride replaces silanol for at least 95% of the available silanol
activity, thus the resulting 0-5% available silanol activity is up to ten
times less than a fully end capped type B phase.
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