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Can Your HPLC Column Do This?
TYPE-C Silica™ Unbonded compared to Cogent UDC Cholesterol™ – Page 17
This slide shows the unique property of TYPE-C Bare Silica ™ to function both in aqueous reverse phase
and organic normal phase. It can be seen that for the organic modifiers typically used in aqueous reverse phase;
methanol is the weakest solvent when compared to acetonitrile and THF. Methanol also gives the worst band-broadening
and worst symmetry of these solvents. The acetonitrile response is a significant improvement over methanol with THF appears
better than the acetonitrile for both parameters. It will be noted, however, that the best symmetry and least band broadening
are achieved when using organic normal phase solvents of hexane and ethyl acetate.
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