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Can Your HPLC Column Do This?
Selectivity Comparison in Aqueous Reverse Phase of Neutrals on Bonded and Un-Bonded
Phases – Page 24
We have seen that both the bare and bonded TYPE-C™ phases can exhibit reverse
phase responses. In the next three slides we will compare the aqueous reverse
phase response of both bare TYPE-C Silica™ and Cogent UDC-Cholesterol™ with TYPE-C™
for target neutral, acid and basic compounds. These analyses are performed at a pH
to elicit a reverse phase response.
When using identical eluents and column sizes plus plotting on the same time scale,
it can be seen that Bare TYPE-C Silica™ and Cogent UDC-Cholesterol™ have different
selectivities for these standards, yet both give sharp symmetrical peaks in reverse
phase mode.
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