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Can Your HPLC Column Do This? What About Phase Collapse With 100% Aqueous on TYPE-C™ Products? – Page 27 The poor performance of reverse phase material, especially C18, after flowing 100% aqueous eluent is well documented. Many, but not all, consider this to be a phase collapse criteria.
Irrespective of its cause, it is important for analysis of polar molecules
to prevent this loss of performance (seen usually as a progressive reduction
of retention times with prolonged exposure to 100% aqueous eluents and also
often a broadening of peaks).
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