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Can Your HPLC Column Do This?
What About Peptides? – Page 31
It is traditionally thought that a 300-angstrom phase is needed for larger peptide
and protein analysis, however, much of this bias is caused by attempts to reduce
silanol activity and bonded phase adsorption by use of large pore size to greatly
reduce surface area.
Microsolv’s research into this field is in its infancy, but preliminary results
suggest that the weak aqueous reverse retention mechanism of certain TYPE-C
Silica™ may prove ideal for larger peptides and proteins and the bonded phases for
small to medium size peptides, shown in the next slides.
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