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How to Condition these Columns for Use

First Things First

The best and most efficient way to condition the TYPE-C™ HPLC Columns is to run approximately 6 bed volumes of the mobile phase you are going to use in your run through the column. It is very important to note that if the column was stored with solvents that might be immiscible with this mobile phase, you must first flow a commonly miscible solvent, such as isopropanol, through the column for 15 bed volumes, prior to re-equilibration in the aqueous or organic based solvent to be next used. Be careful that the back pressure does not become excessive when purging with IPA as this is a more viscous solvent which can generate high back pressures even at low flow rates.

Then you should inject either a known standard or one of the standards used in the actual test chromatogram supplied with the column with appropriate mobile phase or, in your mobile phase and re-run until you get reproducible and acceptable results. If you use one of the standards and the test chromatogram and you cannot reproduce k’ or N/meter, contact us before proceeding and we will advise you on possible remedies.

If you are working with a sample for the first time, it is suggested that you first equilibrate as described above with a compound that is known to you to establish equilibration. Then you should run your unknown at least two times.

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