Peptide Mapping by HPLC
Why Use a Cogent HPS™ C18 Column for Peptide Mapping?
Cogent HPS™ C18 columns are perfectly suited for peptide analysis. The pore diameter, the exhaustive end capping and
the low metal content of the silica make these columns an excellent choice for Peptide Mapping after digestion with trypsin.
Unique selectivity and low cost make these columns the choice of many protein laboratories worldwide when reproducibility
and reliability are mandatory.
Discussion:
An in-process method for qualifying a synthetic peptide is shown with 3 samples injected on the same column. The
reproducibility from run to run on the samples is excellent giving the user a high level of confidence. The process and
method was further confirmed by coupling the HPLC separation directory to Mass Spec. The mass data had a high degree of
accuracy and reproducibility; verifying the peptide.
Baseline drift can be minimized with fresh TFA, for improved UV-chromatography.
